Journal: Advanced Materials (Deerfield Beach, Fla.)

Article Title: Unlocking NIR‐II Photoluminescence in 2D Copper Tetrasilicate Nanosheets through Flame Spray Synthesis

doi: 10.1002/adma.202503159

Figure Lengend Snippet: Photoluminescence engineering of copper tetrasilicates enables emission shift to NIR‐II window. a) NIR emission spectrum of BaCuSi 4 O 10 and its mixed forms showing a significant impact of (multi)element doping toward shifting the emission into the NIR‐II window (> 1000 nm). b) Evaluation of the NIR emission spectra as integrated for NIR‐I (simplified as < 1000 nm) and for NIR‐II (> 1000 nm). c) Simplified energy diagram of Cu 2+ ion within a tetragonally distorted crystal field, for i) non‐doped, single M ‐containing NS and ii) multielement doped NS, highlighting the shifted E a energy levels iii). d) Absolute photoluminescence quantum yield (PL‐QY) spectra of CaCuSi 4 O 10 . Integrated photon counts within the gray box, excitation at 630 nm. e) PL‐QY dependency on the excitation wavelengths (red line = Gaussian fit; PL‐QY = 32%). f) PL‐QY engineering through variation of annealing temperature of resynthesized CTS. g) PL‐QY engineering through optimizing annealing time, showing a general trend of increasing PL‐QY with prolonged annealing (mean ± SD). h) Correlation between the lattice parameters a and c , obtained from Rietveld refinement, and the calculated (mean) ionic radius of the (mixed) alkaline earth metal CTS. i) Correlation of the optimized PL‐QY to the emission wavelengths of all synthesized 2D CTS variations (for comparison with reference values see Figure (Supporting Information), red line = second order polynomial fit). j) Correlation between the PL‐QY and fluorescence lifetime of all obtained materials (red line = second order polynomial fit of non‐Mg containing NS).

Article Snippet: In addition, NIR fluorescence emission spectra were recorded with a NIRQuest+1.7 spectrometer (slit width = 200 μm; InGaAs detector, OceanOptics), fiber‐coupled to a customized Axiovert 40CFL using a 10x objective, 800 nm dichroic mirror (Edmund optics) and 900 nm LP filter (FELH0900, Thorlabs).

Techniques: Synthesized, Comparison, Fluorescence

Journal: Advanced Materials (Deerfield Beach, Fla.)

Article Title: Unlocking NIR‐II Photoluminescence in 2D Copper Tetrasilicate Nanosheets through Flame Spray Synthesis

doi: 10.1002/adma.202503159

Figure Lengend Snippet: Nanosheet annealing through laser irradiation. a) Photograph of primary FSP particles (i; cyan) rearranged into NIR‐fluorescent SrCuSi 4 O 10 (ii; blue) through 808 nm laser irradiation (15.3 W cm −2 , white circle) (scale bar = 0.5 cm). b) Schematic representation of the in situ rearrangement process and XRD pattern of the corresponding particles. The amorphous primary FSP particles anneal within seconds into the characteristic P4/ncc tetragonal CTS crystal lattice, similar to a calcination process at 1000 °C (10 min). c) Fluorescence emission spectra of (multielement doped) CTS obtained by laser irradiation.

Article Snippet: In addition, NIR fluorescence emission spectra were recorded with a NIRQuest+1.7 spectrometer (slit width = 200 μm; InGaAs detector, OceanOptics), fiber‐coupled to a customized Axiovert 40CFL using a 10x objective, 800 nm dichroic mirror (Edmund optics) and 900 nm LP filter (FELH0900, Thorlabs).

Techniques: Irradiation, In Situ, Fluorescence

Journal: Advanced Materials (Deerfield Beach, Fla.)

Article Title: Unlocking NIR‐II Photoluminescence in 2D Copper Tetrasilicate Nanosheets through Flame Spray Synthesis

doi: 10.1002/adma.202503159

Figure Lengend Snippet: Engineered nanosheets for super‐resolution mapping of the murine brain. a) Schematic of the diffuse optical localization imaging (DOLI) system used for cerebrovascular imaging in the NIR window. A SWIR camera was used to collect the fluorescence emission of a dispersion of stabilized NS injected intravenously (i.v.) under 808 nm excitation (850 mW cm −2 ). b) Photostability of CTS NSs compared to a common organic dye (Rhodamine B). c) High‐frame‐rate imaging of CaCuSi 4 O 10 NS placed inside a vessel‐mimicking Teflon tube (280 µm inner diameter). Light scattering of brain tissues was mimicked with a 1.2% intralipid (IL) phantom (scale bar = 500 µm). d) Time‐lapse widefield images post DMSA‐stabilized NS injection (scale bar = 1 mm). e) Differentiation of veins and arteries based on their different perfusion patterns, distinguished through principal component analysis (PCA) (scale bar = 1 mm). f) Schematic overview i) of the working principle of DOLI rendering the structural ii), blood flow direction iii) and velocity iv, mm/s) maps of cerebral vasculature from continuous localization and tracking of circulating PEGylated NSs (scale bar = 1 mm).

Article Snippet: In addition, NIR fluorescence emission spectra were recorded with a NIRQuest+1.7 spectrometer (slit width = 200 μm; InGaAs detector, OceanOptics), fiber‐coupled to a customized Axiovert 40CFL using a 10x objective, 800 nm dichroic mirror (Edmund optics) and 900 nm LP filter (FELH0900, Thorlabs).

Techniques: Imaging, Fluorescence, Dispersion, Injection

Journal: Advanced Materials (Deerfield Beach, Fla.)

Article Title: Unlocking NIR‐II Photoluminescence in 2D Copper Tetrasilicate Nanosheets through Flame Spray Synthesis

doi: 10.1002/adma.202503159

Figure Lengend Snippet: Individual macrophage tracking in vivo. a) Macrophage cell toxicity test for various NS compared to SiO 2 (Aerosil 90; mean ± SD). b) Schematic representation of NSs uptaken by human macrophages, with respective bright field (BF) and NIR‐fluorescence images of a single NS‐labeled cell. c) Overlay of all tracked macrophages (N = 15) resemble parts of the vasculature tree (DOLI image from Figure , Supporting Information; scale bar = 1 mm).

Article Snippet: In addition, NIR fluorescence emission spectra were recorded with a NIRQuest+1.7 spectrometer (slit width = 200 μm; InGaAs detector, OceanOptics), fiber‐coupled to a customized Axiovert 40CFL using a 10x objective, 800 nm dichroic mirror (Edmund optics) and 900 nm LP filter (FELH0900, Thorlabs).

Techniques: In Vivo, Fluorescence, Labeling

Journal: Nature Communications

Article Title: Ultrasound-responsive theranostic platform for the timely monitoring and efficient thrombolysis in thrombi of tPA resistance

doi: 10.1038/s41467-024-50741-y

Figure Lengend Snippet: a Synthesis scheme for hmSi-CREKA-RB-PFH. hmSi hollow mesoporous silica, CREKA Cys-Arg-Glu-Lys-Ala peptide, RB Rose Bengal, PFH perfluorohexane. b TEM image of hmSi-CREKA-RB-PFH. TEM transmission electron microscope. c N 2 adsorption-desorption isotherms of hmSiO 2 (insets: corresponding pore size distribution). d Element mapping for Si, N, I, and F. e UV‒VIS absorption spectra and digital images of different submicron particles. a.u. refers to absorbance unit. 1 O 2 was detected based on f DPBF degradation rate and g changes in SOSG fluorescence intensity. n = 3 independent experiments. Data are presented as mean ± SEM. Source data underlying graph c , e – g are provided as a Source Data file. Each experiment was repeated three times or more independently with similar results.

Article Snippet: The major organs of rats were harvested on day 0 and day 7 after injections for ex vivo fluorescence imaging (Vilber, Newton 7.0 Bio) and H&E staining, including heart, liver, spleen, lung and kidney.

Techniques: Transmission Assay, Microscopy, Adsorption, Pore Size, Fluorescence

Journal: Scientific Reports

Article Title: Spinning-disc confocal microscopy in the second near-infrared window (NIR-II)

doi: 10.1038/s41598-018-31928-y

Figure Lengend Snippet: Comparison of wide-field (WF) and spinning-disc confocal (CF) NIR microscope images. ( a ) Wide-field (left) and confocal (right) images of 186 ± 48 nm NIR fluorescent beads. Scale bar = 1 µm. ( b ) Intensity profiles of the cross-sections indicated in ( a ) by dashed lines. ( c ) Z-stack projection of wide-field (top) and confocal (bottom) images of the fluorescent beads. Scale bar = 2 µm. ( d ) Wavelength dependence of theoretical and determined axial resolutions. The theoretical resolutions were calculated according to Equation (purple dashed line) and Equation for wide-field F z = 0.89 (blue dashed line) and confocal F z = 0.66 (red dashed line) cases, where NA = 1.49, n = 1.515 , , . Empirical resolutions in the wide-field settings (white diamonds) were determined using enhanced yellow fluorescent protein (excitation at 480 nm, emission at 525 nm), quantum dots (excitation at 425 nm, emission at 640 nm), and NIR beads (excitation at 780 nm, emission at 980 nm), and they were compared to empirical data in the literature (shaded diamonds) , . The empirical resolution in the confocal settings (red triangle) was determined using NIR beads (excitation at 780 nm, emission at 980 nm), and it was compared to empirical data in the literature (shaded triangle) .

Article Snippet: FluorophorexTM NIR fluorescent polystyrene beads with a mean diameter of 186 ± 48 nm were purchased from Phosphorex Inc. Beads were diluted to ~3 × 10 10 particles per mL and 5 μL of the sample were spin-coated on a glass coverslip at 3000 rpm for 30 s following sonication (Polos SPIN150i, Semiconductor Production Systems).

Techniques: Comparison, Microscopy

Journal: Scientific Reports

Article Title: Spinning-disc confocal microscopy in the second near-infrared window (NIR-II)

doi: 10.1038/s41598-018-31928-y

Figure Lengend Snippet: Spatiotemporal response of immobilized GOx-SWCNT sensors to glucose. ( a ) Schematic of the setup. GOx-SWCNTs sensors were embedded in a 2% agarose gel within a glass-bottom well. ( b ) Confocal planar images (step size = 25 µm) of representative clusters with NIR fluorescent GOx-SWCNTs (excitation at 660 nm, emission above 980 nm). The observed fluorescence increase of the GOx-SWCNT sensors over time was triggered by adding enough glucose solution to yield a final concentration of 15 mM. Scale bar = 2.5 µm. ( c ) Normalized ((I-I 0 )/I 0 ) fluorescence intensity change of five GOx-SWCNT clusters from each axial position after addition of glucose (empty circles), where I is intensity and I 0 is initial intensity. The lines show average intensity changes at the top (green), middle (red), and bottom (blue) slices after applying locally weighted scatterplot smoothing (LOWESS).

Article Snippet: FluorophorexTM NIR fluorescent polystyrene beads with a mean diameter of 186 ± 48 nm were purchased from Phosphorex Inc. Beads were diluted to ~3 × 10 10 particles per mL and 5 μL of the sample were spin-coated on a glass coverslip at 3000 rpm for 30 s following sonication (Polos SPIN150i, Semiconductor Production Systems).

Techniques: Agarose Gel Electrophoresis, Fluorescence, Concentration Assay